ACUC Standard Operating Procedures (SOP's)

UNBC has developed Standard Operating Procedures (SOP's), that have been approved by the UNBC ACUC in compliance with Canadian Council on Animal Care standards, to streamline the protocol writing process for faculty. Please email if you would like to obtain any copies of the SOP's listed below.

Animal Care, Procedures and Welfare


A1-1 Feeding Laboratory Rodents
A1-2 Rodent Husbandry
A1-3 Watering Laboratory Rodents
A1-4 Rodent Housing
A1-5 Cage Cards and Animal Identification
A1-6 Daily Animal Care
A1-7 Quarantine

Health Care and Animal Welfare

A2-1 Colony Health Surveillance
A2-2 Health Checking
A2-3 Dead Animals
A2-4 Veterinary Care
A2-5 Disease Outbreak
A2-6 Diseases
A2-7 Escaped Animals
A2-8  Housing of Animals Outside of the NHSRF or Aquatic Facility
A2-9 Post Approval Monitoring Program (PAM)
A2-10 Animal Welfare Incident Reporting


A3-1 Aseptic Technique
A3-2 Non-recovery Cranial Window Surgery

Non-surgical Procedures

A4-1 Rodent Restraint 
A4-2 Saphenous Blood Sampling
A4-3 Cardiac Puncture
A4-4 Tail Vein Blood Sampling
A4-5 Facial Vein Blood Sampling
A4-6 Intraperitoneal Injections in Rodents
A4-7 Intramuscular Injections in Rodents
A4-8 Subcutaneous Injections
A4-9 Intravenous Injections in Rodents
A4-10 Oral Gavage in Rodents
A4-11 Glucose Tolerance Test (OGTT)
A4-12 Insulin Tolerance Test (ITT)
A4-13 Tail Docking
A4-14 Maintaining a Transgenic Breeding Colony
A4-15 Polyclonal Antibody Production in Rodents
A4-16 Monoclonal (ascites) Antibody Production in Mice
A4-17 Polyclonal Antibody Production in Rabbits
A4-18 Blood Sampling in Rabbits


A5-1 Injectable Anesthesia
A5-2 Inhalant Anesthesia
A5-3 Assessing Pain in Rodents
A5-4 Distress Scoring Sheet
A5-5 Anesthetic Depth and Monitoring
A5-6 Analgesia in Rodents
A5-7 Local Analgesia / Anesthesia in Rodents
A5-8 Grimace Scale for Assessing Pain


A6-1 Appropriate Endpoints
A6-2 Euthanasia
A6-3 Isoflurane / Carbon Dioxide Euthanasia
A6-4 Injectable Euthanasia

Animal Acquisition and Shipment

A7-1 Ordering Laboratory Rodents
A7-2 Receiving Laboratory Rodents
A7-3 Animal Care Courses and Training
A7-4 Shipping Live Mice Within Canada

Aquatic Facility

AQ-01 Daily Monitoring of Water Quality
AQ-02 Anesthetizing Laboratory Fish
AQ-03 Feeding Juvenile Fish
AQ-04 Determining Endpoints in Fish
AQ-05 Euthanizing Fish
AQ-06 Fish Health Monitoring
AQ-07 Electrical Power Failure
AQ-08 Loss of Potable Water
AQ-09 Strike or Walkout
AQ-10 Personnel Trauma in the Aquatic Facility
AQ-11 Fish Transportation and Receiving


W-01 Euthanasia of Fish During Fieldwork
W-02 Catching and Handling Wild Fish
W-03 Fish Technical Procedures
W-04 Electrofishing
W-05 Use of Electric Gloves
W-06 Anesthesia of Fish During Fieldwork
W-07 Mist Netting
W-08 Handling Wild Birds
W-09 Wild Bird Sample Collection
W-10 Banding Wild Birds
W-11 Euthanasia of Birds During Fieldwork

Containment Level II Procedures

C-01 Working in CL2
C-02 Animal Husbandry in CL2
C-03 CL2 Waste Disposal
C-04 Spills in CL2

Emergency Management

E-01 Crisis management ACUC Policy Statement
E-02 Fire
E-03 Electrical Power Outage
E-04 Lack or Loss of Heating
E-05 Loss of Potable Water
E-06 Personal Trauma/Injury
E-07 Evacuation and Relocation of Research Animals
E-08 Emergency Health Situations
E-09 Animal Activists and Demonstrations
E-10 Union Strike or Walk-out
E-11 Equipment Maintenance and/or Shut Down
E-12 Pandemic Preparedness Plan
E-13 Personnel Safety during Animal Transportation
E-14 Crisis Management Communication
E-15 Fish Emergency Plan (under development)

Health, Safety and Security

F-01 Occupational Health and Safety, Medical Surveillance Program
F-02 Personal Protective Equipment
F-04 Bites and Scratches
F-05 Allergies
F-06 Working Alone
F-07 Security
F-08 Isoflurane Safety